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The immobilization of Bacillus psychrosaccharolyticus nucleoside 2′-deoxyribosyltransferase was deeply investigated and finally optimized. The best immobilization procedure resulted to be ionic adsorption on PEI 600Da agarose followed by crosslinking with 70% oxidized dextran (20kDa). The percentage of recovered activity was further improved (from 21% to 33%) by the addition of 20% glycerol to the...
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