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A simple radiochemical choline acetyltransferase (ChAT) assay screening test was developed by measuring for [ 3 H]acetylcholine ([ 3 H]ACh) formed from 0.2mM [ 3 H]acetyl-coenzyme A ([ 3 H]acetyl-CoA) and 1mM choline by 0.2mg of rat brain homogenates containing ChAT into 96-well microplates. A simple and rapid procedure for isolating [ 3 H]ACh from the incubation...
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