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One new ergostane steroid, ergosta-4,6,8,22E-tetraen-11β-ol (1), together with five known analogues (2–6), was isolated from the fruiting bodies of Coprinus setulosus for the first time. Their structures were elucidated by analysis of 1D and 2D spectroscopic data as well as MS. The inhibitory activity of these isolates against acetylcholinesterase (AChE) showed that compound 1 exhibited weak inhibitory...
Background Overlapping transcription constitutes a common mechanism for regulating gene expression. A major limitation of the overlapping transcription assays is the lack of high throughput expression data. Results We developed a new tool (IAOseq) that is based on reads distributions along the transcribed regions to identify the expression levels of overlapping genes from standard RNA-seq data....
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