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The results here show for the first time that pH and monovalent cations can regulate cytosolic free Ca 2+ in E. coli through Ca 2+ influx and efflux, monitored using aequorin. At pH 7.5 the resting cytosolic free Ca 2+ was 0.2–0.5 µM. In the presence of external Ca 2+ (1 mM) at alkaline pH this rose to 4 µM, being reduced to 0.9 µM at acid pH. Removal of external Ca...
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