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Two isoenzymes of glutamine synthetase (EC 6.3.1.2), GS1 and GS2, have been purified from cells of Emiliania huxleyi using Cibacron blue dye ligand chromatography and gel filtration, separated by ion-exchange chromatography on Mono-Q and partly characterized. Each enzyme is a homohexamer with a molecular mass of 402 kDa for GS1 and 501 kDa for GS2. The molecular mass of the subunits of GS1 and GS2...
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