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NaHCO3 was used to increase the concentration of dissolved inorganic carbon (DIC) for autotrophic microalgal cultivation in open pond systems. The effects of DIC on the growth of Chlorella vulgaris were investigated under different initial DIC concentrations of 17, 33, 62, 123, and 361 mg carbon/L under no mixing condition. NaHCO3 was found to be an excellent buffer that can help increase DIC concentrations...
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