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The harvesting of microbial cells associated with inulinase transmission through membranes by tangential microfiltration was investigated with Candida kefyr media. Using a rotational disc-filter, seven different kinds of membranes were tested: Pall 66/N (0 20 μm), Pall N (0 04 μm), Nuclepore (cut-off of 10 6 Da), ER-02 (0 2 μm), HVLP (0 45 μm), GVWP (0 22 μm) and VVLP (0 10 μm). The...
Inulinase from K. marxianus was extracted into a reversed micelle phase of the cationic surfactant BDBAC (n-benzyl-n-dodecyl-n-bis(2-hydroxyethyl)ammonium chloride) in isooctane/hexanol. The extractions carried out with cells (5.9 g/l) presented a recovery yield of 87% and a purification factor 2.8. Similar values were found for inulinase recovered from the clarified medium (91% recovery yield and...
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