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Currently, hematoxylin–eosin (H–E) stained histopathology is the golden standard for diagnosing lung cancer. This time‐consuming procedure needs tissue biopsy, sample fixation, slicing, and labeling. Therefore, the availability of a noninvasive optical diagnosis that can obtain real‐time analysis comparable to golden standard H–E stained histopathology will be of extraordinary benefit to the medical...
Liver or lung biopsy for suspicious lesions has several disadvantages such as bleeding, bile leak or pneumothorax, needle track seeding, and time‐consuming histopathological procedure. The ability to directly observe cellular and subcellular details and then perform “optical biopsy” is a major goal in the development of new interventional techniques. Multiphoton microscopy (MPM) enables real‐time...
In contrast to colonoscopy biopsy, which contains several disadvantages such as bleeding, sampling error, crush artifact, and time‐consuming pathological procedure, multiphoton microscopy (MPM) enables direct noninvasive visualization of tissue architecture and cell morphology in live tissues without the administration of exogenous contrast agents. We performed a proof‐of‐principle study to evaluate...
Early detection of fibroadenoma (FA) is critical for preventing subsequent breast cancer. In this work, we show that label‐free second harmonic generation (SHG) imaging is feasible and effective in quantitatively differentiating the fibroadenomal tissue from normal breast tissue. With the advent of the clinical portability of miniature SHG microscopy, we believe that the technique has great potential...
In this paper, multiphoton microscopy (MPM), based on two‐photon excited fluorescence and second harmonic generation signals, was used to image microstructures of human rectal mucosa and submucosa. The morphology and distribution of the main components in mucosa layer, goblet cells, intestinal glands, and a little collagen fibers have been clearly monitored, and the content and distribution of collagen,...
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