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Fentanyl is an extremely potent synthetic opioid that is widely used for chronic pain treatment; it is highly addictive and prone to abuse. The objective is to develop a high throughput homogeneous enzyme immunoassay (HEIA) for the rapid detection of fentanyl in human urine.The HEIA is based on an immunoassay format in which both the antibody and enzyme–drug conjugate are in ready-to-use solution...
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