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Directed evolution of farnesyl diphosphate (FPP, C 15 ) synthase (IspA) of Escherichia coli was carried out by error-prone PCR with a color complementation screen utilizing C40 carotenoid pathway enzymes. This allowed IspA mutants with enhanced production of the C40 carotenoid precursor geranylgeranyl diphosphate (GGPP, C 20 ) to be readily identified. Analysis of these mutants was...
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