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A process for production of an exogenous gene or its product in a plant cell which comprises: inserting into a genome of a plant; a) cDNA of replicase gene from RNA plant virus, and b) cDNA of a recombinant virus genomic RNA in which nucleotide moiety at and after ATG downstream from the original translation initiation codon (the first ATG counted from the 5 -end) in the cDNA of coat protein gene...
We have constructed transgenic tobacco plants (M1x2-FCP2IFN plants) expressing viral RNA replication genes of brome mosaic virus (BMV) and BMV RNA3 derivative (FCP2IFN) carrying the human gamma Interferon (IFN-γ) gene. In M1x2-FCP2IFN plants the RNA3 derivative expressed from the integrated cDNA was replicated and subgenomic RNA (i.e. mRNA of IFN-γ) was produced by BMV replicase. The accumulation...
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