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Mit Sternchen: Die selektive Markierung von N‐Glykanen auf der Oberfläche lebender Zellen gelingt mittels exogener rekombinanter ST6Gal‐I‐Sialyltransferase und azidmodifiziertem CMP‐Neu5Ac und anschließender spannungsvermittelter Cycloaddition mit einem biotinmodifizierten Dibenzylcyclooctinol (siehe Schema; roter Stern=Biotin). Mithilfe dieser Methode können die Mechanismen krankheitsbedingt veränderter...
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