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Ubiquitin (Ub)‐conjugating enzymes (E2s) transfer activated Ub from Ub‐activating enzymes (E1s) to substrates and are associated with various cancers and neurological disorders. In this study, the unique properties of E2‐binding and auto‐ubiquitination of artificial RING fingers (ARFs) were demonstrated in ubiquitination assays. Circular dichroism spectra indicated the characteristic structures of...
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