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In this study, we analyzed the diversity of vaginal microbiota in 9 bacterial vaginosis (BV) positive and 11 healthy women from the metropolitan area of Guangzhou in Southern China using full-length 16S rDNA. Our data confirmed that a shift in the abundance of bacterial species present in the vaginal environment comparing the BV and non-BV groups. Each sequence read was assigned to a genus or a species...
Ultrasonic elastography is an imaging technique of obtaining tissue relative stiffness information. This imaging modality has been shown to be useful in different clinical applications such as tumor detection. Many algorithms for elastography have been presented recently; However, most of them are either inefficient for real-time or not robust for general clinical applications. In this paper we develop...
Mechanical force and geometric deformation that are applied on the cell will be transduced into biomedical signals which has been known as mechanotransduction. In turn, we expect that a particular cell function could be realized by giving a specific physical stimulus to the cell. In this work, a novel cell membrane strain model for circular cell membrane under indentation is developed. Based on this...
We have cloned and expressed a novel earthworm fibrinolytic enzyme (EFE) of Lumbricus rubellus in Pichia pastoris. Its cDNA sequence (GenBank Accession No. DQ202401) revealed a 738 bp region containing an intact ORF that encodes a protein of 245 amino acid residues, containing a signal peptide of 7 amino acid residues and a mature peptide of 238 amino acid residues, designated as EFE F238. Its cDNA...
The restriction-modification systems of C. glutamicum impede its uptake of foreign DNA and thus limiting its transformation efficiency. Here we investigated the possibility of improving the transformation efficiency of C. glutamicum by characterizing and circumventing their restriction/modification barrier, as well as by optimizing the conditions of electroporation. We found that, among various conditions...
This paper presents the characteristics and advantages of polymer optical fiber (POF) sensors compared with common silica optical fiber (SOF) sensors. The development and application of POF sensors for strain measurement are introduced in detail. Because of the recent developments in POF technology, the future of POF sensors is considered optimistic.
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