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Highly sensitive detection of proteins is essential to biomedical research as well as clinical diagnosis. However, usual detecting methods are complicated operating, time-costing and extensive label preparing. Here, we combined molecular beacon (MB) track mediated DNA walker and nicking enzyme assisted signal amplification method to develop a simple and ultrasensitive malachite green fluorescence...
MicroRNA (miRNA) has become an important biomarker candidate for cancer diagnosis, prognosis, and therapy. In this study, we have developed a novel fluorescence method for sensitive and specific miRNA detection via duplex specific nuclease (DSN) signal amplification and demonstrated its practical application in biological samples. Malachite green (MG) was employed as a “label-free" signal transducer...
Imaging of microRNA (miRNA) in living cells could facilitate the monitoring of the expression and distribution of miRNA and research on miRNA-related diseases. Given the low expression levels and even down-regulation of cellular miRNA that is associated with some diseases, enzyme-free amplification strategies are imperative for intracellular miRNA assay. In this work, we report an entropy-driven reaction...
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