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To investigate the ability of the retinoblastoma gene product (RB protein, pRB) to regulate its own expression, cotransfection assays using human RB promoter-luciferase fusion plasmids and a human pRB expression plasmid were employed. In B104, a rat neuroblastoma cell line, pRB stimulated luciferase activity about 2-fold from the wild-type promoter, and about 4-fold from a mutant promoter with a...
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