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This paper reports on a biomimetic microskeleton actuated by muscle fibers (figure 1(a)). The 3D microskeleton is based on concatenated rib elements fabricated by two-photon polymerization of a custom-made hydrogel (figure 1(b)). For the first time, micrometer-sized skeletons were fabricated, overgrown with functional muscle fibers, and contracted at significant amplitudes. Compared to planar biomimetic...
We developed a portable odorant detection system composed of collagen micro-pillars with cells expressing olfactory receptors and a CMOS imaging sensor; the CMOS imaging sensor caught fluorescence signals of Ca2+ increased by odorant molecules. While low sensitiveness of the CMOS imaging sensor was insufficient to detect the fluorescent signal from a single cell, the odorant detection system achieved...
Transmembrane proteins functioning as the cellular signaling gateway are the major molecular targets of commercial drug therapy. HEK293T cells are world widely used cell based-platform for these proteins analysis. We loaded the photo-curable hydrogel into the HEK293T cells, treated with UV through the micron photo-mask, and fabricated the cell membrane fiber. The activity of ion channels on the surface...
This paper describes a contactless manipulation system for giant liposomes in an aqueous flow by negative dielectrophoresis (nDEP). The system consists of 2 pairs of comb electrodes that face each other across a perpendicular channel. This design allows an nDEP force to be focused at the center of the gap of the electrodes and the liposomes to align in lines without contacting either the electrodes...
This paper describes a preliminary report of microfabricated dissolvable mobile microplates for handling adherent cells. We fabricated micron-sized (10∼100 μm) alginate hydrogel plates by soft lithography, attached adherent cells, and handled them. In contrast to previous microplates [1–3], the alginate microplates could be dissolved without damaging cells. We demonstrated dissolution of microplates...
We aimed to create a method in micropatterning of different kinds of biomaterials as a platform of a molecular communication system onto a single substrate. This paper proposes a multiple poly(para-xylylene) (parylene) simultaneous peel-off process and demonstrates that different kinds of proteins and DNAs were successfully microarrayed onto the single substrate. Further, the functionalities and the...
This letter describes the integration of an electrical connection onto a lipid bilayer membrane microchip for the electrical detection/regulation of transmembrane phenomena together with fluorescence monitoring. Arrayed microchambers with a volume of 3 pL were fabricated within a poly(dimethyl siloxane) microfluidic device, and electrical access was achieved by designing channels adjacent to the chambers...
This paper describes a method to transplant neurospheroid network onto the rat brain. We first patterned the uniform sized neurospheroids on the PDMS microchamber, and cultured for 1-2 weeks. After 2-week culture, the neurospheroids tightly connected each other with extending their neuronal processes (e.g. dendrite, axons) and formed neuronal network. We realized that the neurospheroid network can...
This paper describes core-shell hydrogel wires for constructing 3D heterogeneous hydrogel microstructures containing biomaterials. A core hydrogel layer contains biomaterials such as cells, and a shell hydrogel layer covers the core to realize a mechanically-robust hydrogel wire. Using this core-shell gel wires, we demonstrate a woven sheet with heterogeneous gel wires by using our stereolithographically-made...
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