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The current techniques for cell cutting always use a diamond knife with an edge angle larger than 20°. The compression caused by the edge angle may destroy the sample. In this paper, a nano knife with a small edge angle 5° was fabricated from a commercial AFM cantilever by FIB etching. The mechanical character of the nano knife was calibrated by theoretical calculation, numerical simulation and nanorobotic...
Cell-cell adhesion force is important for cell activities. In this paper, a novel cell-cell adhesion force measurement method using nano picker was proposed based on the nanorobotic manipulation system inside an environmental scanning electron microscopy (ESEM). The nano picker was fabricated from atomic force microscopy(AFM) cantilever by focused ion beam(FIB) etching. The spring constant of the...
We propose a buckling nanoneedle as a force sensor for stiffness characterization of single cells. There are notable advantages of using buckling nanoneedle for single cells stiffness characterizations such that severe cell damage from an excessive indentation force could be prevented and large variations in single cells stiffness property could be easily detected either from the dented mark on the...
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