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Cell-cell adhesion force is important for cell activities. In this paper, a novel cell-cell adhesion force measurement method using nano picker was proposed based on the nanorobotic manipulation system inside an environmental scanning electron microscopy (ESEM). The nano picker was fabricated from atomic force microscopy(AFM) cantilever by focused ion beam(FIB) etching. The spring constant of the...
In this paper, the local stiffness of alive Caenorhabditis elegans (C. elegans) is evaluated by environmental-scanning electron microscope (E-SEM) nanorobotic manipulation system. The local and surface stiffness of C. elegans is important to evaluate to inject or insert micro-nano probes for gene-deliver or electrophysiologic applications. At first, we observe their fine structures by E-SEM system...
In this work, a nano injection system for single cell with small size (diameter: ~5 ??m) is proposed. An environmental scanning electron microscope (E-SEM) nanorobotic manipulation system has been constructed with three units and seven degrees of freedom (DOFs) in total. It can provide a real-time observation with nanoscale resolution during the experiment. Micro pulling and FIB etching are used to...
This paper reports the technique of metal nanoelectrode growth on the tip of carbon nanotube by field emission. The nanorobotic manipulation system inside a FE-SEM was used for this experiment. Depending on the diameter of the positive electrode, the density of electric field can be controlled for nanowire growth by field emission. By introducing the different precursors in chamber, different metal...
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