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This paper presents the evaluation of ultra-minimal spout amount from micro-nano pipettes into phospholipid-coated micro-droplets. The pipettes can be used to control the local environment around/inside single cells. Conventionally, the microinjection with pipette has been conducted by air pressures. In this method, the spout of high viscosity solutions is difficult because of the frictional forces...
We propose a nano size clock, capsulated into micro size liposome, made by protein molecules. The circadian clock is a basic cellular system found in almost all organisms. This clock generates self-sustained oscillations under constant conditions with a ??24-hour (circadian) period. In cyanobacteria, circadian clock was reconstituted by only three proteins. We reconstitute these proteins and adenosine...
Single cell analysis gets a lot of attentions to reveal the unknown biological aspects of individual cells. To analyze properties of a single cell, local environmental control is desired. We propose a novel type of nanopipette where a lipid nanotube (LNT) as a nanochannel is attached to apply the minimal changes to the environment. LNTs have hollow cylindrical nanostructures consisting of lipid bilayer...
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