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In this paper, a new strategy is proposed for measuring the cell adhesion force by using a nanofork and a line-patterned substrate. The nanofork has three tines which resembles the appearance of a normal fork. In order to effectively implement the nanofork, a special design substrate, known as line-patterned (LP) substrate, is required. The spaces between the line structures on the substrate provide...
Cell-cell adhesion force is important for cell activities. In this paper, a novel cell-cell adhesion force measurement method using nano picker was proposed based on the nanorobotic manipulation system inside an environmental scanning electron microscopy (ESEM). The nano picker was fabricated from atomic force microscopy(AFM) cantilever by focused ion beam(FIB) etching. The spring constant of the...
In this paper, single cell adhesion force was measured using a nanofork. The nanofork was used to pick-up a single cell on a line-patterned substrate inside ESEM. The line-patterned substrate was used to provide small gaps between the single cells and the substrate. Therefore, the nanofork could be inserted through these gaps in order to successfully pick-up a single cell. Adhesion force was measured...
We present the environmental-SEM (ESEM)-nanomanipulator system for in-situ measurement of mechanical properties of the yeast cells (W303 strain). This enhanced ESEM system comprises of a standard ESEM instrument as a nano imaging tool, a cooling stage as a cellular biology's humidity controller and 7 D.O.F. linear actuators as nanomanipulator/effector. Two types of experiments have been conducted,...
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