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We report the design of an oligonucleotide sensor for the detection of point mutation associated with sickle cell disease. The sensor was based on luminescence resonance energy transfer between a donor and an acceptor. Photon upconverting nanoparticles (NaYF 4 doped with Yb 3+ and Er 3+ ) were used as the donor and a conventional fluorophore, N,N,N′,N′-tetramethyl-6-carboxyrhodamine...
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