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Impedance measurements provide basic electrical properties and are used to analyze the characteristics of electrochemical materials for biomedical applications. The extracellular fluid (ECF) in microfluidic devices greatly affects the accuracy of impedance measurements of cells. When a single cell is placed in large amounts of ECF, the electric current mostly passes through the ECF, not the cell....
This work presents an electrical impedance spectroscopy (EIS) impedance analysis system for a single HeLa cell measurement in the frequency range of 11 to 101 kHz. The system includes a micro-controller-unit (MCU) using 8051F320 chip, an impedance analyzer using AD5934 chip, a function generation circuit and a biochip utilizing electric trapping method in a compact device. The biochip utilizes the...
This paper presents the development and application of a miniaturized impedance sensing system for a single cell impedance measurement. The system includes a set of plane electrodes on a biochip, a function generator, an impedance analyzer and a handheld microscope. The system also equips a programmable controller for the automatic impedance measurement and analysis. It can execute the cell located...
When an electrical current with a low frequency is applied to a cell, the current passes through the outside of the cell. Thus, impedance measurements at low frequencies cannot be used to determine the pathological change of the cellular organelle taking place inside the cell. However, increasing the frequency of the electrical current makes the capacitive impedance of the cell decrease, allowing...
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