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An enzyme-based glucose/O 2 biofuel cell was constructed within a microfluidic channel to study the influence of electrode configuration and fluidic channel height on cell performance. The cell was composed of a bilirubin oxidase (BOD)-adsorbed O 2 cathode and a glucose anode prepared by co-immobilization of glucose dehydrogenase (GDH), diaphorase (Dp) and VK 3 -pendant poly-l-lysine...
Viamin K 3 -modified poly-l-lysine (PLL-VK 3 ) was synthesized and used as the electron transfer mediator during catalytic oxidation of NADH by diaphorase (Dp) at the anode of biofuel cell. PLL-VK 3 and Dp were co-immobilized on an electrode and then coated with NAD + -dependent glucose dehydrogenase (GDH). The resulting enzymatic bilayer (abbreviated PLL-VK 3 ...
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