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To examine second messenger pathways involved in neurokinin induced bladder contractions. Neurokinin induced changes in inositol phosphate production and in adenylyl cyclase activity are measured in the guinea pig bladder. Substance P, substance P methyl ester, neurokinin A, and neurokinin B each increase [sup 3 H]-inositol phosphate production in the guinea pig bladder. Substance P (10 sup...
Muscarinic cholinergic and adrenergic agonist-induced changes in [sup 3 H]-phosphatidyl inositol (PI) hydrolysis and cyclic AMP (cAMP) levels were measured in guinea pig ureter, urethra and bladder dome. In the ureter, carbachol, norepinephrine and phenylephrine rapidly increased PI hydrolysis and basal cAMP levels, but did not decrease forskolin-stimulated cAMP levels. In the bladder dome, norepinephrine...
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