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A new approach for monitoring the binding affinity between drugs and alpha 1-acid glycoprotein in real time was developed based on a combination of drug–protein reaction followed by Venturi easy ambient sonic-spray ionization mass spectrometry determination of the free drug concentrations. A known basic drug, propranolol was used to validate the new built method. Binding constant values calculated...
Pillar[5]arenes bearing ten phosphine oxide groups (1a–e), as analogs of their corresponding calix[4]arene-based phosphine oxide, have demonstrated intriguing recognition performance for some representative heavy metal cations including Co 2+ , Cu 2+ , Ni 2+ , Zn 2+ , Cd 2+ , Pb 2+ , Ag 2+ and Hg 2+ compared to their acyclic species (2a–e)...
Metabolomics is the downstream of systems biology and has drawn significant interest for studying the metabolic networks from cells to organisms. To profile the metabolites in two different cell lines (A549 and AGS) infected with influenza A virus, gas chromatography coupled with mass spectrometry (GC/MS) was employed. Some differentiating metabolites in the cell lines were tentatively identified...
Nucleoprotein (NP), the structural component of ribonucleoprotein complex of avian influenza virus, performs multiple essential functions in the regulation of viral RNA synthesis and in the control of nuclear traffic of viral proteins. Mutations have often been found in NP, some of which are relevant to viral survival strategies. In this study, we used nanospray-MS/MS to analyze tryptic digestion...
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