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H9N2 subtype low pathogenicity avian influenza virus (AIV) poses a potential zoonotic risk. PA‐X, a novel protein generated by PA gene ribosomal frameshift, is considered to be the virulence factor of H9N2 subtype AIVs. Our study found that rTX possessing PA‐X protein enhanced the mammalian pathogenicity of H9N2 subtype AIVs compared with PA‐X‐deficient virus (rTX‐FS). Furthermore, PA‐X protein inhibited...
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