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Conventional neuronal analysis at the single neuron level usually involves culturing of neurons in vitro and analysis of neuronal activities by electrophysiological or pharmacological methods. However, the extracellular environments of in vitro neuronal analysis cannot mimic the exact surroundings of the neurons. Here, we report a microfluidic worm-chip for in vivo analysis of neuronal activities...
We demonstrate a microfluidic system allowing stable immobilization of young adult C. elegans for neuronal and behavior analysis upon chemical stimulations. Chemical stimuli were delivered to the head of the animal by rapid interface shifting of laminar flows and neuronal activities could then be monitored by in vivo Ca2+ imaging.
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