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The urea-induced dissociation of nerve growth factor from venom of Chinese cobra (cNGF) was studied by intrinsic fluorescence emission spectra, SEC, urea-gradient polyacrylamide gel electrophoresis, assays of biological activity and thermodynamic parameters. The results showed that when urea concentration was lower than or equal to 4.0 mol L−1 or higher than or equal to 8.0 mol L−1, cNGF existed only...
The present experiments were designed for the development of a liquid chromatographic coupled with tandem mass spectrometric method for the quantitation of trace amount of protoveratrine A in rat plasma. A liquid-liquid extraction protocol was involved in sample preparation. Quantitation was achieved using multiple-reaction-monitoring (MRM) mode. The method was validated with the concentration range...
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