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Objectives: The aim of the study is to test the hypothesis that tension-dependent change in the affinity of cardiac troponin-C influences the time courses of Ca 2+ transients and tension in twitch contraction. Methods: The Ca 2+ -sensitive photoprotein, aequorin, was microinjected into superficial cells of ferret papillary muscles and the Ca 2+ transients and...
Objective: We investigated the effects of thapsigargin (TG) (0.1-1 μM) on the relation between intracellular Ca 2+ concentration and tension in ferret papillary muscles using aequorin-injected and skinned preparations. Methods: Aequorin was injected into the superficial cells of ferret papillary muscles; the Ca 2+ signals of aequorin and tension in twitch and those with the...
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