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In this paper, we presented a design for an automated single cell loading and supply system that can be used with complex microfluidic applications requiring single cell loading such as the current nuclear transplantation method. The aim of the system is to automatically transfer mammalian donor (~15 ??m) or egg (~100 ??m) cells one by one from a container to a PDMS micro-channel and then transport...
We have proposed cell coupling system for mammalian somatic cell cloning process in microfluidic chips. There are two kinds of nuclear transfer methods, microinjection and cell fusion; we selected the cell fusion method. Unlike the conventional method by the manual labor under the microscope, our aim is developing automated cloning work which is being performed in microfluidic chip. Although this...
The interest in dynamically tuning lens has attracted great attention to fabricate compact vision systems. Here, we discuss the fabrication and characterization of a simple, tunable, yet fully disposable, except imaging sensor, microfluidic-based optical device. The desired compact system is structured on a PDMS microfluidic. Tunable lens actuated by flow pressure (2 mm in diameter) is controlled...
Recently, micro valves to control some kinds of fluids in channels in a microchip have been developed. Now we pay attention to in-chip somatic cell cloning with microfluidic technology. There are some steps, cell supply, cutting, sorting, coupling, fusion and so on, for this work. And we focused on a cell supply system spending cells one by one in microfluidic chip, and attempt to transport an individual...
In this paper, we presented a design for an automated cell supply system that can be used with complex microfluidic applications requiring single cell loading such as the current nuclear transplantation method. The aim of the system is to automatically transfer mammalian donor (~15 ??m) or egg (~100 ??m) cells one by one from a container to a PDMS micro-channel and then transport them to other modules...
Automated continuous individual cell transfer is a critical step in single-cell applications using microfluidic devices. Cells must be aspirated gently from a buffer before transferring to operation zone so as not to artificially perturb their biostructures. Vision-based manipulation is a key technique for allowing nondestructive cell transportation. In this paper, we presented a design for an automated...
This paper describes research and development of a compact vision system for real-time cell detection and manipulation in a capillary in order to apply on the "automated nuclear transplantation" project which will achieve sort of cell manipulation tasks such as positioning, cutting, sizing, and so on, automatically between different interconnected modules. Here, we propose a vision system...
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