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A technology based on laser-induced fluorescence combined with microstructured fiber for micrometer-size sample detection and collection is presented. The fiber-based system introduces the possibility for real-time monitoring in in-vivo measurements.
An all-fiber capillary electrophoresis system is presented. It enables sensitive in-line electrophoresis separation and fluorescence detection. As a proof of concept, a biological sample (FITC-BSA) is electrokinetically separated and analyzed.
The standard Ar ion laser in a confocal microscope was replaced with an intra-cavity frequency doubled Nd:YAG laser operating at 473 nm. The fluorescence image quality suggests that excitation at 473 nm could be preferable.
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