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Prion diseases are transmissible spongiform encephalopathies caused by deleterious prion protein (PrPSc) derived from normal prion protein (PrPC), which is encoded by the prion protein gene (PRNP). We performed an in‐depth examination to detect PrPSc by using enzyme immunoassay (EIA), real‐time quaking‐induced conversion reactions (RT‐QuIC) and protein misfolding cyclic amplification (PMCA) in nine...