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The phospholipase D (PLD) gene pld encoding the mature PLD enzyme fromStreptomyces chromofuscus was cloned and sequenced. The recombinant protein has been expressed inEscherichia coli and purified. The yield of aim protein was up to 27.5 mg/l culture medium. Because it was targeted with a 6 × his-tag, the recombinant protein could be much more easily purified by using Ni-NTA His·Bind Purification...
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