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The introduction and subsequent expression of foreign DNA inside living mammalian cells (transfection) is achieved by photoporation with a violet diode laser. We direct a compact 405 nm laser diode source into an inverted optical microscope configuration and expose cells to 0.3 mW for 40 ms. The localized optical power density of ~1200 MW/m2 is six orders of magnitude lower than that used in femtosecond...
Stable transfection of Chinese hamster ovary (CHO-K1) cells is achieved by photoporation with a violet diode laser. This represents the simplest method of laser-assisted cell poration reported to date
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