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To construct a simple model cellular system exhibiting the property of self-propelled motion, cell-sized giant liposomes encapsulating desired amounts of actoHMM, a mixture of actin filament (F-actin) and heavy meromyosin (HMM, an actin-related molecular motor), have been prepared. We adapted the methodology of spontaneous transfer of a water droplet through oil/water interface in the presence of...
Using a spontaneous transfer method, cell-sized giant liposomes encapsulating desired amounts of actoHMM, a mixture of actin filament (F-actin) and heavy meromyosin (HMM, an actin-related molecular motor), have been successfully constructed in the presence of 5 mM MgCl2 and 50 mM KCl. The encapsulated actoHMM formed self-organized actin network-like structures, and non-spherical liposomes were obtained...
We confined actin and giant DNA mixture in a phospholipid coated micrometer scale (cell size) closed space (PMS) as a cell nuclear model system. We report the appearance of a spatially segregated state in PMSs between actin filaments and giant DNA molecules. When the diameter of a PMS was below 15 ??m, DNA molecules are excluded toward the surface by forming an assembly with actin filaments. Furthermore,...
It has been established that a long DNA molecule (> 20-30 kilo base-pairs) exhibits a conformational transition from a coiled state to a highly folded state under the presence of condensing agents such as polyamines. We have investigated these changes in conformation of long DNA molecules at a single-molecule level by directly observing conformation of individual DNA molecules in the bulk aqueous...
An in vitro translation system, based on cell components of the hyperthermophilic archaeon, Thermococcus kodakaraensis, has previously been developed. The system has been optimized and applied for protein production at high temperatures (60-65??C). In this paper, we have examined the possibilities to utilize this system at a lower temperature range using green fluorescence protein (GFP) as the reporter...
Nanoparticles, as nonviral vectors, have attracted attention for their ability in gene delivery and are currently considered to be prospective future vectors for biological and biomedical application . We prepared organically modified cationic silica nanoparticles toward the high performance nonviral vector. It is to be noted that the complexes of DNA and nanoparticles are regarded as a simple model...
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