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The possibilities of an in vitro translation system, based on cell components of the hyperthermophilic archaeon, Thermococcus kodakaraensis, to utilize at a lower temperature range were examined. Green fluorescence protein (GFP) was used as the reporter protein. Through optimization of several experimental conditions, the yield of active GFP production at 40??C increased 5-fold. The optimized system...
The emerging field of synthetic biology seems to have a great potential to the development of new biotechnologies and the understanding of self-organizing principle in life. Currently, several synthetic biologists aim to understand the evolution of naturally occurring biological systems through a "bottom-up approach" in contrast to the conventional reductive approach. Several lines of pioneering...
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