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Gene encoding a novel translation initiation factor PeIF5B from Pisumsativum with sequence similarity to eIF5B from H.sapiens, D.melanogaster, S. cerevisiae as well as archaeal aIF5B from M.thermoautotrophicum was earlier reported by us. We now describe the expression and purification of 96 kDa recombinant PeIF5B (rPeIF5B) protein. Using fluorescence and circular dichroism spectra analyses, we...
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