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By using iodide (I − ) as a quencher, we successfully improve the fluorescence response of amiloride when binding to thymine opposite an AP site in a 21-meric DNA duplex. From fluorescence measurements, as compared to the NaCl solutions, the addition of NaI as a quencher as well as salt to adjust the ionic strength effectively suppresses the background fluorescence from unbound amiloride in...
An automatic system, based on the on-line coupling of high-performance liquid chromatography (HPLC) separation, post column microwave digestion, and cold-vapor atomic fluorescence spectrometry (CVAFS) detection, was proposed for the speciation analysis of four mercury compounds. Post column microwave digestion, in the presence of potassium persulfate (in HCl), was applied in the system to improve...
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