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The development of a modified carbon paste electrode incorporating the enzyme tyrosinase (EC 1.14.18.1) and a surfactant (sodium hexadecanesulfonate) permits to operate in media containing high proportions of an organic solvent (acetonitrile or methanol). The choice of the enzyme has been exploited to assay phenol and catechol derivatives of pharmaceutical interest at the micromolar concentration...
The performance of a tyrosinase-modified solid carbon paste electrode (SCPE) as electrochemical detector has been studied in comparison with a glassy carbon electrode detector in high-performance liquid chromatography for the simultaneous determination of dopamine, 3,4-dihydroxyphenylacetic acid, norepinephrine and homovanillic acid. The influence of pH, flow-rate and amount of organic solvent...
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