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A novel method is described for site-specific and chemical crosslinking of oligodeoxynucleotides containing 6-methylsulphoxypurine with cysteine or peptides containing cysteine. 6-Methylsulphoxy group on purine is stable in aqueous solution, but easily replaceable by the thiol group of cysteine and glutathione. A mechanism of the reaction is proposed, and potential applications are discussed with...
A convenient and economical method is described for the site-specific 15 N-labelling of the 4-amino group of individual cytidine residues in oligonucleotides. This is applied to a 20 base-pair oligonucleotide corresponding to the trp operator; this oligonucleotide and its complex with the E. coli trp repressor are studied by NMR, using 1 H- 15 N HMQC and 1 ...
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