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Transmigration of human granulocytes across a basal lamina equivalent was studied in vitro. Transwell(R) inserts were coated with Matrigel(R), a reconstituted basement membrane. Granulocytes (2x10 6 ) were applied to the upper chamber. As chemoattractant interleukin-8 (IL-8; 25 ng/ml) was added to the lower chamber. After 1 h of migration, cells were counted in the lower chamber. Specific...
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