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An experimental study was carried out to evaluate the performance of a polydimethylsiloxane (PDMS) based micromixer for heterogeneous immunoassays of insulin. The detection method is based on coupling the highly specific double-antibody sandwich immunoassay with the sensitive chemiluminescence of the Luminol-Hydrogen Peroxide reaction. Both the immune and chemiluminescence reactions are conducted...
The bioconjugation efficiency resulting from three PDMS based continuous flow micromixers are experimentally studied at various sample flow rates and pneumatic actuation frequencies. In this study, streptavidin-coupled paramagnetic microbeads and biotin-coupled fluorescent microbeads are separately injected into the micromixers and the resultant binding efficiency is measured by the fluorescence emission...
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