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A progressive increase in the response of a nociceptive spinal neuron to repeated electrical C-fiber stimulation reflects a phenomenon called windup. Second order neurons in the dorsal horn, as well as motoneurons, can develop windup. Inhaled anesthetics act primarily in spinal cord to suppress movement induced by noxious stimulation. We hypothesized that halothane would depress neuronal windup in...
We describe a method to record single unit neuronal activity from mouse spinal cord using volatile anesthesia. The small size of the mouse can complicate usual methods that are used for single-unit recording in rats, but simple modifications can significantly increase the number of successful recordings. Stabilization of the vertebral column is particularly important, as are adequate ventilation of...
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