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Mechanisms underlying acetylcholine-induced endothelium-independent vasodilation were studied in the rat mesenteric vascular bed isolated from Wistar rats. In preparations without endothelium, and contracted by perfusion with Krebs solution containing methoxamine (2-7 μM), perfusion of acetylcholine (1-100 μM) for 1 min produced a concentration-dependent vasodilation. Denervation of denuded preparations...
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