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A series of eight experiments was conducted to determine the most suitable cryoprotectants (CPAs), dilution rates and methods of CPA addition as well as freezing method for the cryopreservation of Greenshell™ mussel (Perna canaliculus) sperm. The experiments utilized a fertilization assay to determine the treatments that gave the highest post-thaw fertility. The assays revealed that dimethyl sulphoxide...
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