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A semi-quantitative PCR-based system has been developed for detection of DNA sequences for the soybean allergen Gly m Bd 30K. The selected primers were highly specific for soybean and did not show amplification from a panel of legume relatives. Repeatability was assessed in a spiking experiment of soybean in wheat flour (0.0001–100% soybean), using known standards for comparison of the amount of output...
The introduction of novel proteins into foods carries a risk of eliciting allergic reactions in individuals sensitive to the introduced protein. Therefore, decision trees for evaluation of the risk have been developed, the latest being proposed by WHO/FAO early in 2001. Proteins developed using modern biotechnology and derived from fish are being considered for use in food and other applications,...
The aim of the study was to standardize and evaluate technically optimized food allergen extracts for use in skin prick test (SPT). The standardization procedure comprised 36 allergic histories in 32 food allergic patients with 21 healthy, non-atopic individuals serving as controls. The patients had a history of allergic symptoms upon ingestion of either cow's milk (n=3), hen's egg (n=9), wheat (n=4),...
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