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Here, we report the volume and enthalpy changes accompanying CO photodissociation from the mixed valence form of cytochrome bo 3 oxidase from Escherichia coli. The results of photoacoustic calorimetry indicate two kinetic phases with distinct volume and enthalpy changes accompanying CO photodissociation from heme o 3 and its transfer to Cu B . The first phase occurring on a...
In this report we describe the activation volumes associated with the heme–heme electron transfer (ET) and CO rebinding to the binuclear center subsequent to photolysis of the CO-mixed-valence derivative of Escherichia coli cytochrome bo3 (Cbo). The activation volumes associated with the heme–heme ET (k=1.2×105 s−1), and CO rebinding (k=57 s−1) are found to be +27.4 ml/mol and −2.6 ml/mol, respectively...
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