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Bacillus cereus, Listeria monocytogenes, and Staphylococcus aureus caused problems in public health and food safety. A multiplex real‐time PCR (qPCR) for simultaneous detection of these three pathogens in different kinds of food was developed. A high specificity (100%) was obtained using 21 target strains and 19 nontarget strains. Standard curves for pure cultures covered seven orders of magnitude...
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