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A synthetic gene for apoleghemoglobin-a from soybean, optimized for expression in Escherichia coli has been designed and synthesized by a recursive polymerase chain reaction technique. The protein has been expressed with high efficiency and a purification protocol has been developed. The holoprotein is readily reconstituted by the addition of heme. 15 N- and 15 N, 1...
A 25-residue synthetic peptide corresponding to zinc finger 31 of the Xenopus protein Xfin adopts a compact, folded conformation in the presence of zinc. Complete 1 H resonance assignments have been made. The peptide contains a helix, beginning as an α-helix and ending as a 3 10 -helix, that extends from residue 12 to 23. Several positively charged and polar residues located on...
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